We made a new glutamate indicator with improved sensitivity, kinetics & localization for imaging synaptic transmission.
This video is DIV12 rat cortical culture with 2uM TTX, imaged with a simple widefield microscope (NA1.4) at 100Hz, displayed here at 20Hz. Flashes correspond to spontaneous release of the neurotransmitter glutamate, monitored using iGluSnFR3.
Glutamate detection using genetically encoded indicators has come a long way! Here’s a stroll down memory lane showing the development of glutamate biosensors over the past two decades.
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